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rat lncrna/mrna microarray  (Arraystar inc)

 
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    Arraystar inc rat lncrna/mrna microarray
    Rat Lncrna/Mrna Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat lncrna/mrna microarray/product/Arraystar inc
    Average 90 stars, based on 1 article reviews
    rat lncrna/mrna microarray - by Bioz Stars, 2026-06
    90/100 stars

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    The expression of RBM4 in cardiomyocyte is mediated by <t>m6A-YTHDF1</t> (A) The potential m6A sites in RBM4 mRNA predicted by SRAMP. (B) The m6A methylation of RBM4 mRNA in cardiomyocyte is increased by Ang II stimulation. The N6-methylated RNAs captured from cardiomyocytes treated with Ang II or saline (con) by m6A antibody or control IgG, were purified with TRIzol. The RBM4 mRNA level was analyzed by qPCR. ****P < 0.0001. n = 3. (C) The levels of m6A writer or eraser proteins in cardiomyocytes were analyzed by immunoblotting. n = 3. (D) The interaction between RBM4 mRNA and YTHDF1 was increased by Ang II stimulation. The RNA captured from the lysates of cardiomyocytes treated with Ang II or saline (con) by YTHDF1 antibody or control IgG was purified with TRIzol. The RBM4 mRNA level was analyzed by qPCR. *P < 0.05, **P < 0.01, ***P < 0.001. n = 3. (E,F) YTHDF1 enhances RBM4 translation during cardiomyocyte hypertrophy. Cardiomyocytes were transfected with control siRNA or YTHDF1 siRNA and then treated with Ang II. (E) The protein levels of RBM4 and YTHDF1 were analyzed by immunoblotting. (F) The RBM4 mRNA level was analyzed by qPCR. ns, P ≥ 0.05. n = 3.
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    The expression of RBM4 in cardiomyocyte is mediated by m6A-YTHDF1 (A) The potential m6A sites in RBM4 mRNA predicted by SRAMP. (B) The m6A methylation of RBM4 mRNA in cardiomyocyte is increased by Ang II stimulation. The N6-methylated RNAs captured from cardiomyocytes treated with Ang II or saline (con) by m6A antibody or control IgG, were purified with TRIzol. The RBM4 mRNA level was analyzed by qPCR. ****P < 0.0001. n = 3. (C) The levels of m6A writer or eraser proteins in cardiomyocytes were analyzed by immunoblotting. n = 3. (D) The interaction between RBM4 mRNA and YTHDF1 was increased by Ang II stimulation. The RNA captured from the lysates of cardiomyocytes treated with Ang II or saline (con) by YTHDF1 antibody or control IgG was purified with TRIzol. The RBM4 mRNA level was analyzed by qPCR. *P < 0.05, **P < 0.01, ***P < 0.001. n = 3. (E,F) YTHDF1 enhances RBM4 translation during cardiomyocyte hypertrophy. Cardiomyocytes were transfected with control siRNA or YTHDF1 siRNA and then treated with Ang II. (E) The protein levels of RBM4 and YTHDF1 were analyzed by immunoblotting. (F) The RBM4 mRNA level was analyzed by qPCR. ns, P ≥ 0.05. n = 3.

    Journal: Acta Biochimica et Biophysica Sinica

    Article Title: Endogenous RBM4 prevents Ang II-induced cardiomyocyte hypertrophy via downregulating the expression of PTBP1

    doi: 10.3724/abbs.2024103

    Figure Lengend Snippet: The expression of RBM4 in cardiomyocyte is mediated by m6A-YTHDF1 (A) The potential m6A sites in RBM4 mRNA predicted by SRAMP. (B) The m6A methylation of RBM4 mRNA in cardiomyocyte is increased by Ang II stimulation. The N6-methylated RNAs captured from cardiomyocytes treated with Ang II or saline (con) by m6A antibody or control IgG, were purified with TRIzol. The RBM4 mRNA level was analyzed by qPCR. ****P < 0.0001. n = 3. (C) The levels of m6A writer or eraser proteins in cardiomyocytes were analyzed by immunoblotting. n = 3. (D) The interaction between RBM4 mRNA and YTHDF1 was increased by Ang II stimulation. The RNA captured from the lysates of cardiomyocytes treated with Ang II or saline (con) by YTHDF1 antibody or control IgG was purified with TRIzol. The RBM4 mRNA level was analyzed by qPCR. *P < 0.05, **P < 0.01, ***P < 0.001. n = 3. (E,F) YTHDF1 enhances RBM4 translation during cardiomyocyte hypertrophy. Cardiomyocytes were transfected with control siRNA or YTHDF1 siRNA and then treated with Ang II. (E) The protein levels of RBM4 and YTHDF1 were analyzed by immunoblotting. (F) The RBM4 mRNA level was analyzed by qPCR. ns, P ≥ 0.05. n = 3.

    Article Snippet: Double-strand small interfering RNA (siRNA) oligonucleotides specific for rat RBM4 mRNA, PTBP1 mRNA and YTHDF1 mRNA were designed and purchased from GenePharma Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Methylation, Saline, Control, Purification, Western Blot, Transfection